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Intranuclear Trafficking of Fluorescent Hiv-1 Viral Particles

HIV-1 preferentially integrates in transcriptionally active regions of the host cellular chromatin independently from strong consensus sequences. To investigate whether the nuclear architecture may affect viral integration we have developed an experimental system where HIV-1 viral particles can be visualized within the nuclear compartment. Fluorescently labeled HIV-1 virions were engineered by fusing integrase, the viral protein that catalyzes the integration reaction, to fluorescent proteins. 3-D confocal microscopy allowed a detailed analysis of the spatial and temporal distribution of the pre-integration complexes (PICs) within the nucleus at different moments following infection; the fluorescently labeled PICs preferentially distribute in decondensed areas of the chromatin and in the nuclear periphery, while heterochromatin regions are largely disfavored. These observations provide a first indication of how the nuclear architecture may initially orient the selection of retroviral integration sites.

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2. CHAPTER 2 MATERIAL AND METHODS

Tesi di Dottorato

Dipartimento: Biologia Molecolare

Autore: Alberto Albanese Contatta »

Composta da 196 pagine.

 

Questa tesi ha raggiunto 147 click dal 29/10/2009.

Disponibile in PDF, la consultazione è esclusivamente in formato digitale.