Implementation of a Chemiluminescent Immunoassay for S. Aureus Protein A in a Cd Microfluidic Device

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3 divalent cations (at higher concentrations of bound α – toxin). In the latter case, small molecules can also pass through the pore.  β – toxin. This toxin is an sphingomyelinase which damages lipid-rich membranes. The majority of isolates from bovine mastitis express this toxin, suggesting that this toxin is important for the pathogenesis of mastitis.  δ – toxin. This small peptide is produced by most strains of S. aureus, S. epidermidis and S. lugdunensis, but its role is still unknown.  γ – toxin and leukocidin. These two toxins are expressed separately but act together in attacking membranes of susceptibles cells.  Coagulase which binds to prothrombin forming a complex called staphylothrombin. Coagulase is a traditional marker for identifying S. Aureus in clinical specimens. Recently it has been shown that coagulase can bind fibrinogen as well as thrombin.  Staphylokinase, a plasminogen activator. The complex between staphylokinase and plasminogen affects plasmin-like proteolytic activity, which causes dissolution of fibrin clots. However, as for coagulase, there is no evidence that this protein represebts a virulence factor.  S. Aureus also expresses proteases, lipases, deoxyribonucleases and a fatty acid modifying enzyme (FAME) that might be important in the onset of abscesses modifying anti-bacterial lipids and prolonging bacterial survival (Cuong 2002). 1.2 PROTEIN A The Staphylococcal Protein A (or simply protein A) is a monomeric protein, which is covalently bound to the cell wall peptidoglycan of most strains of S. aureus. Its exact molecular weight is unknown, and reported values vary from 41000 (obtained by physical methods such as equilibrium sedimentation or gel chromatography under denaturing conditions) to 55000-56000 (obtained by SDS-polyacrylamide gel electrophoresis). The protein is bound to the peptidoglycan of the bacterium trough its COOH-terminal part and interacts with the Fc-fragment of immunoglobulins by its NH 2 – terminal part.

Anteprima della Tesi di Deborah Sebastianelli

Anteprima della tesi: Implementation of a Chemiluminescent Immunoassay for S. Aureus Protein A in a Cd Microfluidic Device, Pagina 4

Laurea liv.II (specialistica)

Facoltà: Farmacia

Autore: Deborah Sebastianelli Contatta »

Composta da 53 pagine.

 

Questa tesi ha raggiunto 25 click dal 29/10/2012.

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